From 03fc8c8b650ef93cf4031661a5b69d1e05c76113 Mon Sep 17 00:00:00 2001 From: "Erica Y. Scott" Date: Wed, 25 Mar 2020 12:10:21 -0400 Subject: [PATCH] Update README.md --- README.md | 18 +++++++++++++----- 1 file changed, 13 insertions(+), 5 deletions(-) diff --git a/README.md b/README.md index 4f0bb48..dbf6bb0 100644 --- a/README.md +++ b/README.md @@ -3,11 +3,19 @@ DISCO-Digital Microfluidic Isolation of Single Cells for - Omics The laser lysis capture method implemented in DISCO offers spatially and temporally resolved capture of single cells, while Digital Microfluidics (DMF) is capable of retrieving this lysate and permitting flexible downstream analyses, such as scRNA-seq. Each cell has a barcode incorporated into Read 1, making the cell's transcriptome accountable and traceable through the pipeline. This barcode includes a unique cell barcode (12bp) and a UMI (8bp), the first part of our bioinformatic pipeline deals with parsing these. -##General Worlflow +## General Worlflow The provided scripts go through mapping, parsing fasta headers (barcodes and UMIs), generating count data, making figures and performing differential gene expression (DGE): +1. +2. +3. +4. +5. +FigureScripts: +DependentScripts: -##Publication +## Publication pending -###Authors of scripts -Erica Y. Scott: Scripts 1-5 and figure scripts 1-6 -Harrison Edwards: barcode parser scripts () + +### Authors of scripts +Erica Y. Scott: Scripts 1-5 and Figure scripts 1-6 +Harrison Edwards: python barcode parser scripts within DependentScripts/ -- GitLab