From 22e3279479a45eba4756cb8182bc93a28d2ded9f Mon Sep 17 00:00:00 2001 From: "Erica Y. Scott" Date: Wed, 25 Mar 2020 13:31:18 -0400 Subject: [PATCH] Update README.md --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 48fd581..80f0de1 100644 --- a/README.md +++ b/README.md @@ -3,7 +3,7 @@ DISCO-Digital Microfluidic Isolation of Single Cells for - Omics. The laser lysis capture method implemented in DISCO offers spatially and temporally resolved capture of single cells, while Digital Microfluidics (DMF) is capable of retrieving this lysate and permitting flexible downstream analyses, such as scRNA-seq. Each cell has a barcode incorporated into Read 1, making the cell's transcriptome accountable and traceable throughout the pipeline. This barcode includes a unique cell barcode (12bp) and a UMI (8bp). The first part of our bioinformatic pipeline deals with parsing these. -## General Worlflow +## General Workflow The provided scripts go through mapping, parsing fasta headers (barcodes and UMIs), generating count data, making figures and performing differential gene expression (DGE): 1. Append Read1 barcode information to Read2 fasta header 2. Align with Star Aligner -- GitLab