diff --git a/README.md b/README.md index a0ad1db07529589d630035c07a0a3550cc93041a..5d1d99625d19c95f91c7891d3f06b8ad79bc0370 100644 --- a/README.md +++ b/README.md @@ -1,4 +1,4 @@ -# DISCO: scRNAseq ![alt text](https://github.com/eyscott/DISCO_scRNAseq/blob/master/Images/WheelerLabLogo.png=50x50 "Wheeler Lab") +# DISCO: scRNAseq ![alt text](https://github.com/eyscott/DISCO_scRNAseq/blob/master/Images/WheelerLabLogo.png =50x50 "Wheeler Lab") DISCO-Digital Microfluidic Isolation of Single Cells for - Omics The laser lysis capture method implemented in DISCO offers spatially and temporally resolved capture of single cells, while Digital Microfluidics (DMF) is capable of retrieving this lysate and permitting flexible downstream analyses, such as scRNA-seq. Each cell has a barcode incorporated into Read 1, making the cell's transcriptome accountable and traceable through the pipeline. This barcode includes a unique cell barcode (12bp) and a UMI (8bp), the first part of our bioinformatic pipeline deals with parsing these.