From a9272ddcf51d49d7543136e831c83efa5acf6333 Mon Sep 17 00:00:00 2001 From: "Erica Y. Scott" Date: Wed, 25 Mar 2020 12:07:33 -0400 Subject: [PATCH] Create README.md --- README.md | 13 +++++++++++++ 1 file changed, 13 insertions(+) create mode 100644 README.md diff --git a/README.md b/README.md new file mode 100644 index 0000000..4f0bb48 --- /dev/null +++ b/README.md @@ -0,0 +1,13 @@ +# DISCO_scRNAseq +DISCO-Digital Microfluidic Isolation of Single Cells for - Omics +The laser lysis capture method implemented in DISCO offers spatially and temporally resolved capture of single cells, while Digital Microfluidics (DMF) is capable of retrieving this lysate and permitting flexible downstream analyses, such as scRNA-seq. +Each cell has a barcode incorporated into Read 1, making the cell's transcriptome accountable and traceable through the pipeline. This barcode includes a unique cell barcode (12bp) and a UMI (8bp), the first part of our bioinformatic pipeline deals with parsing these. + +##General Worlflow +The provided scripts go through mapping, parsing fasta headers (barcodes and UMIs), generating count data, making figures and performing differential gene expression (DGE): + +##Publication +pending +###Authors of scripts +Erica Y. Scott: Scripts 1-5 and figure scripts 1-6 +Harrison Edwards: barcode parser scripts () -- GitLab