From afb8ee483a87b7935f7e2fcf1a64d988d37e3f2b Mon Sep 17 00:00:00 2001 From: "Erica Y. Scott" Date: Wed, 25 Mar 2020 21:43:46 -0400 Subject: [PATCH] Update README.md --- README.md | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/README.md b/README.md index fb6eba4..6927df3 100644 --- a/README.md +++ b/README.md @@ -1,4 +1,5 @@ -# DISCO: scRNAseq ![alt text](https://github.com/eyscott/DISCO_scRNAseq/blob/master/Images/WheelerLabLogo.png "Wheeler Lab") +# DISCO: scRNAseq Wheeler Microfluidics Lab + DISCO-Digital Microfluidic Isolation of Single Cells for - Omics. The laser lysis capture method implemented in DISCO offers spatially and temporally resolved capture of single cells, while Digital Microfluidics (DMF) is capable of retrieving this lysate and permitting flexible downstream analyses, such as scRNA-seq. Each cell has a barcode incorporated into Read 1, making the cell's transcriptome accountable and traceable throughout the pipeline. This barcode includes a unique cell barcode (12bp) and a UMI (8bp). The first part of our bioinformatic pipeline deals with parsing these. -- GitLab