Digital microfluidics for cell culture and analysis

In vitro cell culture and analysis is omnipresent in modern biology labs, but this comes at a cost, with world-wide activities requiring an annual expenditure of billions of dollars and hundreds of thousands of laboratory hours. Our group was the first to explore the compatibility of digital microfluidics with mammalian cell culture to automate the process and reduce reagent consumption; papers in this area are listed below. For more, see our recent review paper describing the use of DMF for cell culture and analysis.

Li, B.B.; Scott, E.Y.; Olafsen, N.E.; Matthews, J.; Wheeler, A.R. "Analysis of the Effects of Aryl Hydrocarbon Receptor Expression on Cancer Cell Invasion via Three-Dimensional Microfluidic Invasion Assays" Lab Chip 2022, 22, 313–325. Supporting Info.

Sklavounos, A.A.; Nemr, C.R.; Kelley, S.O.; Wheeler, A.R. "Bacterial Classification and Antibiotic Susceptibility Testing on an Integrated Microfluidic Platform" Lab Chip 2021, 21, 4208 - 4222. Supporting Info.

Lamanna, J.; Scott, E.Y.; Edwards, H.S.; Chamberlain, M.D.; Dryden, M.D.M.; Peng, J.; Mair, B.; Lee, A.; Chan, C.; Sklavounos, A.A.; Heffernan, A.; Abbas, F.; Lam, C.; Olson, M.E.; Moffat, J.; Wheeler, A.R. "Digital Microfluidic Isolation of Single Cells for -Omics" Nature Comm., 2020, 11, 5632. Supplementary: Info, Movie 1, Movie 2.

Li, B.B.; Scott, E.Y.; Chamberlain, M.D.; Duong, B.T.V.; Zhang, S.; Done, S.J.; Wheeler, A.R. "Cell Invasion in Digital Microfluidic Microgel Systems" Science Advances. 2020, 6, eaba9589. Supporting Info.

Yu, Y.; Campos, R.P.S.d.; Hong, S.; Kraste, D.L.; Sadanand, S.; Leung, Y.; Wheeler, A.R. "A Microfluidic Platform for Continuous Monitoring of Dopamine Homeostasis in Dopaminergic Cells" Microsyst. Nanoeng. 2019, 5, 10. Supporting info.

Yu, Y.; Shamsi, M.H.; Krastev, D.L.; Dryden, M.D.M.; Leung, Y.; Wheeler, A.R. "A Microfluidic Method for Dopamine Uptake Measurements in Dopaminergic Neurons" Lab Chip 2016, 16, 543-552. Supporting info.

Ng, A.H.C.; Chamberlain, M.D.; Situ, H.; Lee, V.; Wheeler, A.R. "Digital microfluidic immunocytochemistry in single cells" Nat. Commun., 2015, 6, 7513. Supplementary: Info, Movie 1, Movie 2, Movie 3, Movie 4.

Au, S.H.; Chamberlain, M.D.; Mahesh, S.; Sefton, M.V.; Wheeler, A.R. "Hepatic organoids for microfluidic drug screening" Lab on a Chip, 2014, 14, 3290-3299. Supporting info.

Shih, S.C.C.; Mufti, N.S.; Chamberlain, M.D.; Kim, J.; Wheeler, A.R. "A droplet-based screen for wavelength-dependent lipid production in algae" Energy & Environmental Science, 2014, 7, 2366-2375. Supporting info, supporting video.

Eydelnant, I.A.; Li, B.B.; Wheeler, A.R. "Microgels on-DemandNat. Commun. 2014, 5, 3355. Supplementary: Figures, Movie 1, Movie 2.

Au, S.H.; Fobel, R.; Desai, S.P.; Voldman, J.; Wheeler, A.R. "Cellular bias on the microscale: probing the effects of digital microfluidic actuation on mammalian cell health, fitness and phenotype" Integr. Biol. 2013, 5, 1014. Supporting info.

Shih, S.C.C.; Barbulovic-Nad, I.; Yang, X.; Fobel, R.; Wheeler, A.R. "Digital Microfluidics with Impedance Sensing for Integrated Cell Culture and Analysis" Biosens. Bioelectron. 2013, 42, 314–320. Supporting info

Bogojevic, D.; Chamberlain, M.D.; Barbulovic-Nad, I.; Wheeler, A.R. "A Digital Microfluidic Method for Multiplexed Cell-Based Apoptosis Assays" Lab Chip 2012, 12, 627-634.

Srigunapalan, S.; Eydelnant I.A.; Simmons, C.A.; Wheeler, A.R. "A Digital Microfluidic Platform for Primary Cell Culture and Analysis" Lab Chip 2012, 12, 369-375.

Au, S.H.; Shih, S.C.C.; Wheeler, A.R. "Integrated Microbioreactor for Culture and Analysis of Bacteria, Algae and YeastBiomed. Microdev., 2011, 13, 41-50.

Barbulovic-Nad, I.; Au, S.; Wheeler, A.R. "A Microfluidic Platform for Complete Mammalian Cell CultureLab Chip 2010, 10, 1536-1542. Supporting Info 

Barbulovic-Nad, I.; Yang, H.; Park, P.S.; Wheeler, A.R. "Digital Microfluidics for Cell-Based Assays" Lab Chip 2008, 8, 519-526.