Wheeler Microfluidics Laboratory

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Publications

Publications are posted on these pages to ensure timely dissemination of scholarly and technical work. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's or publisher's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder. For our most recent covers, click here.

Jump to Book Chapters and Review Papers

Primary Publications:

  1. Elsayed, M.; Bodo, L.; Gaoiran, C.; Keuhnelian, P.; Dosajh, A.; Luk, V.; Schwandt, M.; French, J.L.; Ghosh, A.; Erickson, B.; Charlesworth, A.G.; Millman, J.; Wheeler, A.R. "Toward Analysis at the Point of Need: A Digital Microfluidic Approach to Processing Multi-Source Sexual Assault Samples" Advanced Science 2024, 11, 2405712. Supporting Info. Movie.
  2. Casasbuenas, D.L.; Kortebi, I; Gora, C.; Scott, E.Y.; Gomes, C.; Oliviera Jr., M.S.; Sharma, T.; Daniele, E.; Gibbs, R.; Yuzwa, S.A.; Gilbert, E.A.; Küry, P.; Wheeler, A.R.; Lévesque, M.; Faiz, M. "The laminar position, morphology, and gene expression profiles of cortical astrocytes are influenced by tume of birth from ventricular/subventricular progenitors" Glia 2024, 1-14. Supporting Figures. Movie. Supporting Data 1. Supporting Data 1.
  3. Scott, E.Y.; Safarian, N.; Casasbuenas, D.L.; Dryden, M.D.M.; Tockovska, T.; Ali, S.; Peng, J.; Daniele, E.; Lim, I.N.X.; Bang, K.A.; Tripathy, S.; Yuzwa, S.A.; Wheeler, A.R.; Faiz, M. "Integrating Single-Cell and Spatially Resolved Transcriptomic Strategies to Survey the Astrocyte Response to Stroke in Male Mice" Nature Comm. 2024, 15, 1584. Supporting Info.Supporting Data.
  4. Ho, M.; Sathishkumar, N.; Sklavounos, A.A.; Sun, J.; Yang, I.; Nichols, K.P.; Wheeler, A.R. "Digital Microfluidics with Distance-Based Detection – a New Approach for Nucleic Acid Diagnostics" Lab Chip 2024, 24, 63-73. Supporting Info.
  5. Chan, C.; Peng, J.; Rajesh, V.; Scott, E.Y.; Sklavounos, A.A.; Faiz, M.; Wheeler, A.R. "Digital Microfluidics for Microproteomic Analysis of Minute Mammalian Tissue Samples Enabled by Photocleavable Surfactant" J. Prot. Res. 2023, 22, 3242-3253. Supporting Info,Supporting Figures,Movie.
  6. Peng, J.; Chan, C.; Zhang, S.; Sklavounos, A.A.; Olson, M.E.; Scott, E.Y.; Hu, Y.; Rajesh, V.; Li, B.B.; Chamberlain, M.D.; Zhang, S.; Peng, H.; Wheeler, A.R. "All-in-One Digital Microfluidics Pipeline for Proteomic Sample Preparation and Analysis" Chemical Science 2023, 14, 2887 - 2900. Supporting Info 1,Supporting Info 2,Movie.
  7. Chen, X.; Chen, X.; Elsayed, M.; Edwards, H.; Liu, J.; Peng, Y.; Zhang, H.P.; Zhang, S.; Wei Wang; Wheeler, A.R. "Steering Micromotors via Reprogrammable Optoelectronic Paths" ACS Nano 2023, 17, 5894-5904. Supporting Info,Movie 1,Movie 2,Movie 3,Movie 4,Movie 5
  8. Peng, J.; Chan, C.; Meng, F.; Hu, Y.; Chen, L.; Lin, G.; Zhang, S.; Wheeler, A.R. "Comparison of Database Searching Programs for the Analysis of Single-Cell Proteomics Data" J. Prot. Res. 2023, 22, 1298-1308. Supporting Info.
  9. Jenne, A.; von der Ecken, S.; Moxley-Paquette, V.; Soong, R.; Swyer, I.; Bastawrous, M.; Busse, F.; Bermel, W.; Schmidig, D.; Kuehn, T.; Kuemmerle, R.; Adwan-Stojilkovic, D.A.; Graf, S.; Frei, T.; Monette, M.; Wheeler, A.R.; Simpson, A.J. "Integrated Digital Microfluidics NMR Spectroscopy: A Key Step toward Automated in vivo Metabolomics" Anal. Chem. 2023, 95, 5858−5866. Supporting Info.
  10. Ho, M.; Au, A.; Flick, R.; Vuong, T.V.; Sklavounos, A.A.; Swyer, I.; Yip, C.M.; Wheeler, A.R. "Antifouling Properties of Pluronic and Tetronic Surfactants in Digital Microfluidics" ACS Appl. Mater. Interfaces, 2023, 15, 6326−6337. Supporting Info,Movie.
  11. Knipes, A.K.; Summers, A.; Sklavounos, A.A.; Lamanna, J.; de Campos, R.P.S.; Narahari, T.; Dixon, C.; Fobel, R.; Ndjakani, Y.D.; Lubula, L.; Magazani, A.; Muyembe, J.J.; Lay, Y.; Pukuta, E.; Waku-Kouomou, D.; Hao, L.; Kayembe, J.K.; Fobel, C.; Dahmer, J.; Lee, A.; Ho, M.; Valenzuela, J.G.C.; Rackus, D.G.; Shih, R.; Seale, B.; Chang, A.; Paluku, G.; Rota, P.A.; Wheeler, A.R.; Scobie, H.M. "Use of a Rapid Digital Microfluidics-Powered Immunoassay for Assessing Measles and Rubella Infection and Immunity in Outbreak Settings in the Democratic Republic of the Congo" PLOS One, 2022, 17, e0278749. Supporting Info.
  12. Au, A.; Ho, M.; Wheeler, A.R.; Yip, C.M. "Monitoring non-specific adsorption at solid–liquid interfaces by supercritical angle fluorescence microscopy" Rev. Sci. Inst., 2022, 93, 113707. Supporting Info.
  13. Mennillo, L.; Bendkowski, C.; Elsayed, M.; Edwards, H.; Zhang, S.; Pawar, V.; Wheeler, A.R.; Stoyanov, D.; Shaw, M. "Adaptive Autonomous Navigation of Multiple Optoelectronic Microrobots in Dynamic Environments" IEEE Robot. Autom. Lett., 2022, 7, 11102-11109.
  14. Peng, J.; Sun, J.; Yang, M.I.; Gibson, R.M.; Arts, E.J.; Olabode, A.S.; Poon, A.F.Y.; Wang, X.; Wheeler, A.R.; Edwards, E.A.; Peng, H. "Early Warning Measurement of SARS-CoV‑2 Variants of Concern in Wastewaters by Mass Spectrometry" Environ. Sci. Technol. Lett. 2022, 9, 638-644.Supporting Info.
  15. von der Ecken, S.; Sklavounos, A.A.; Wheeler, A.R. "Vertical Addressing of 1-Plate Electrodes for Digital Microfluidics" Adv. Mater. Tech., 2022, 7, 2101251. Supplementary: Info, Movie, Zip file
  16. Narahari, T.; Dahmer, J.; Sklavounos, A.; Kim, T.; Satkauskas, M.; Clotea, I.; Ho, M.; Lamanna, J.; Dixon, C.; Rackus, D.G.; Silva, S.J.R.d.; Pena, L.; Pardee, K.; Wheeler, A.R. "Portable Sample Processing for Molecular Assays: Application to Zika Virus Diagnostics" Lab Chip 2022, 22, 1748-1763. Supporting Info.
  17. Zhang, S.; Elsayed, M.; Peng, R.; Chen, Y.; Zhang, Y.; Neale, S.L.; Wheeler, A.R "Influence of Light Pattern Thickness on the Manipulation of Dielectric Microparticles by Optoelectronic Tweezers" Photonics Research 2022, 10, 550-556. Supplementary Movie.
  18. Li, B.B.; Scott, E.Y.; Olafsen, N.E.; Matthews, J.; Wheeler, A.R. "Analysis of the Effects of Aryl Hydrocarbon Receptor Expression on Cancer Cell Invasion via Three-Dimensional Microfluidic Invasion Assays" Lab Chip 2022, 22, 313–325. Supporting Info.
  19. Shuzhang Liang; Gan, C.; Dai, Y.; Zhang, C.; Bai, X.; Zhang, S.; Wheeler, A.R.; Chen, H.; Feng, L. "Interaction between Positive and Negative Dielectric Microparticles/Microorganism in Optoelectronic Tweezers" Lab Chip 2021, 21, 4379–4389.
  20. Sklavounos, A.A.; Lamanna, J.; Modi, D.; Gupta, S.; Mariakakis, A.; Callum, J.; Wheeler, A.R. "Digital Microfluidic Hemagglutination Assays for Blood Typing, Donor Compatibility Testing, and Hematocrit Analysis" Clin. Chem. 2021, 67, 1699–1708. Supplementary: Info, Movie.
  21. Sklavounos, A.A.; Nemr, C.R.; Kelley, S.O.; Wheeler, A.R. "Bacterial Classification and Antibiotic Susceptibility Testing on an Integrated Microfluidic Platform" Lab Chip 2021, 21, 4208 - 4222. Supporting Info.
  22. Zhang, S.; Li, W.; Elsayed, M.; Peng, J.; Chen, Y.; Zhang, Y.; Zhang, Y.; Shayegannia, M.; Dou, W.; Wang, T.; Sun, Y.; Kherani, N.P.; Neale, S.L.; Wheeler, A.R. "Integrated Assembly and Photopreservation of Topographical Micropatterns" Small , 2021, 17, 2103702. Supporting info, Supporting video
  23. Zhang, S.; Elsayed, M.; Peng, R.; Chen, Y.; Zhang, Y.; Peng, J.; Li, W.; Chamberlain, M.D.; NIkitina, A.; Yu, S.; Liu, X.; Neale, S.L.; Wheeler, A.R. "Reconfigurable Multi-Component Micromachines Driven by Optoelectronic Tweezers" Nature Comm. , 2021, 12, 5349. Supporting info, Supporting video
  24. Peng, R.; Pan, Y.; Liu, B.; Li, Z.; Pan, P.; Zhang, S.; Qin, Z.; Wheeler, A.R.; Tang, X.S.; Liu, X "Understanding Carbon Nanotube-Based Ionic Diodes: Design and Mechanism" Small, 2021, 17, 2100383. Supplementary: Info.
  25. Lamanna, J.; Scott, E.Y.; Edwards, H.S.; Chamberlain, M.D.; Dryden, M.D.M.; Peng, J.; Mair, B.; Lee, A.; Chan, C.; Sklavounos, A.A.; Heffernan, A.; Abbas, F.; Lam, C.; Olson, M.E.; Moffat, J.; Wheeler, A.R. "Digital Microfluidic Isolation of Single Cells for -Omics" Nature Comm., 2020, 11, 5632. Supplementary: Info, Movie 1, Movie 2.
  26. Sathyanarayanan, G.; Haapala, M.; Dixon, C.; Wheeler, A.R.; Sikanen, T.M. "A Digital-to-Channel Microfluidic Interface via Inkjet Printing of Silver and UV Curing of Thiol–Enes" Adv. Mater. Tech. , 2020, 5, 2000451. Supporting info. Supporting video.
  27. Peng, R.; Pan, Y.; Li, Z.; Zhang, S.; Wheeler, A.R.; Tang, X.S.; Liu, X. "Ionotronics Based on Horizontally Aligned Carbon Nanotubes" Adv. Func. Mater. 2020, 30, 2003177.
  28. Li, B.B.; Scott, E.Y.; Chamberlain, M.D.; Duong, B.T.V.; Zhang, S.; Done, S.J.; Wheeler, A.R. "Cell Invasion in Digital Microfluidic Microgel Systems" Science Advances. 2020, 6, eaba9589. Supporting Info.
  29. Dixon, C.; Lamanna, J.; Wheeler, A.R. "Direct loading of blood for plasma separation and diagnostic assays on a digital microfluidic device" Lab on a Chip. 2020, 20, 1845-1855. Supplementary: Info, Movie.
  30. Zhang, S.; Zhai, Y.; Peng, R.; Shayegannia, M.; Flood, A.G.; Qu, J.; Liu, X.; Kherani, N.P.; Wheeler, A.R. "Assembly of Topographical Micropatterns with Optoelectronic Tweezers" Adv. Optical Mater. 2019, 7(20), 1900669. Supplementary: Info, Movie.
  31. Wu, B.; von der Ecken, S.; Swyer, I.; Li, C.; Jenne, A.; Vincent, F.; Schmidig, D.; Kuehn, T.; Beck, A.; Busse, F.; Stronks, H.; Soong, R.; Wheeler, A.R.; Simpson, A. "Rapid Chemical Reaction Monitoring by Digital Microfluidics-NMR: Proof of Principle Towards an Automated Synthetic Discovery Platform" Angew. Chem. Int. Ed. 2019, 58, 15372-15376. Supporting Info.
  32. Zhang, S.; Li, W.; Elsayed, M.; Tian, P.; Clark, A.W.; Wheeler, A.R.; Neale, S.L. "Size-scaling effects for microparticles and cells manipulated by optoelectronic tweezers" Optics Letters, 2019, 44 (17), 4171-4174. Supplementary: Movie 1, Movie 2, Movie 3.
  33. Zhang, S.; Scott, E.Y.; Singh, J.; Chen, Y.; Zhang, Y.; Elsayed, M.; Chamberlain, M.D.; Shakiba, N.; Adams, K.; Yu, S.; Morshead, C.M.; Zandstra, P.W.; Wheeler, A.R. "The optoelectronic microrobot: A versatile toolbox for micromanipulation" Proceedings of the National Academy of Science, U.S.A. 2019, 116 (30), 14823-14828. Supporting Info, Supplementary Movies.
  34. Rahbar, M.; Wheeler, A.R.; Paul, B.; Macka M. "Ion-Exchange Based Immobilization of Chromogenic Reagents on Microfluidic Paper Analytical Devices" Analytical Chemistry 2019, 91, 8756-8761. Supporting Info.
  35. Swyer, I.; Fobel, R.; Wheeler, A.R. "Velocity Saturation in Digital Microfluidics" Langmuir 2019, 35, 5342-5352. Supplementary: Info, Movie 1, Movie 2, Movie 3.
  36. Yu, Y.; Campos, R.P.S.d.; Hong, S.; Kraste, D.L.; Sadanand, S.; Leung, Y.; Wheeler, A.R. "A Microfluidic Platform for Continuous Monitoring of Dopamine Homeostasis in Dopaminergic Cells" Microsyst. Nanoeng. 2019, 5, 10. Supplementary:Supporting info.
  37. Swyer, I.; Ecken, S.v.d.; W, B.; Jenne, A.; Soong, R.; Vincent, F.; Schmidig, D.; Frei, T.; Busse, F.; Stronks, H.J.; Simpson, A.J.; Wheeler, A.R. "Digital Microfluidics and Nuclear Magnetic Resonance Spectroscopy for in situ Diffusion Measurements and Reaction Monitoring" Lab Chip 2019, 19, 641-653. Supporting info.
  38. Campos, R.P.S.d.; Darius G. Rackus; Shih, R.; Chen Zhao; Liu, X.; Wheeler, A.R. "'Plug-n-Play' Sensing with Digital Microfluidics" Anal. Chem. 2019, 91, 2506−2515. Supporting info.
  39. Zhang, S.; Shakiba, N.; Chen, Y.; Zhang, Y.; Tian, P.; Singh, J.; Chamberlain, M.D.; Satkauskas, M.; Flood, A.G.; Kherani, N.P.; Yu, S.; Zandstra, P.W.; Wheeler, A.R. "Patterned Optoelectronic Tweezers: A New Scheme for Selecting, Moving, and Storing Dielectric Particles and Cells" Small, 2018, 45, 1803342. Supplementary: Info, Movie 1, Movie 2, Movie 3, Movie 4, Movie 5, Movie 6.
  40. Ng, A.H.C.; Fobel, R.; Fobel, C.; Lamanna, J.; Rackus, D.G.; Summers, A.; Dixon, C.; Dryden, M.D.M.; Lam, C.; Ho, M.; Mufti, N.S.; Lee, V.; Asri, M.A.M.; Sykes, E.A.; Chamberlain, D.; Joseph, R.; Ope, M.; Scobie, H.M.; Knipes, A.; Rota, P.A.; Marano, N.; Chege, P.M.; Njuguna, M.; Nzunza, R.; Kisangau, N.; Kiogora, J.; Karuingi, M.; Burton, J.W.; Borus, P.; Lam, E.; Wheeler, A.R."A Digital Microfluidic System for Serological Immunoassays in Remote Settings" Sci. Trans. Med. 2018, 10, eaar6076. Supplementary Materials.
  41. Zhang, S.; Nikitina, A.; Chen, Y.; Zhang, Y.; Liu, L.; Flood, A.G.; Juvert, J.; Chamberlain, M.D.; Kherani, N.P.; Neale, S.L.; Wheeler, A.R. "Escape from an optoelectronic tweezer trap: experimental results and simulations" Optics Express, 2018, 26, 5300-5309. Supplementary: Movie 1, Movie 2; Movie 3; Movie 4.
  42. Rackus, D.G.; P. S. de Campos, R.; Chan, C.; Karcz, M.M.; Seale, B.; Narahari, T.; Dixon, C.; Chamberlain, M.D.; Wheeler, A.R. "Pre-Concentration by Liquid Intake by Paper (P-CLIP): A New Technique for Large Volumes and Digital Microfluidics" Lab on a Chip, 2017, 17, 2272-2280. Supporting info.
  43. Abdulwahab, S.; Ng, A.H.C.; Chamberlain, M.D.; Ahmado, H.; Behan, L.-A.; Gomaa, H.; Casper, R.F.; Wheeler, A.R. "Towards a Personalized Approach to Aromatase Inhibitor Therapy: A Digital Microfluidic Platform for Rapid Analysis of Estradiol in Core-Needle-Biopsies" Lab on a Chip, 2017, 17, 1594-1602.
  44. Dixon, C.; Ng, A.H.C.; Fobel, R.; Miltenburg, M.B.; Wheeler, A.R. "An Inkjet Printed, Roll-Coated Digital Microfluidic Device for Inexpensive, Miniaturized Diagnostic Assays" Lab on a Chip, 2016, 16, 4560-4568. Supporting info. Supporting video.
  45. Swyer, I.; Soong, R.; Dryden, M.D.M.; Fey, M.; Maas, W.E.; Simpson, A.; Wheeler, A.R. "Interfacing Digital Microfluidics with High-Field Nuclear Magnetic Resonance Spectroscopy" Lab on a Chip, 2016, 16, 4424-4435. Supporting info.
  46. Seale, B.; Lam, C.; Rackus, D.G.; Chamberlain, M.D.; Liu, C.; Wheeler, A.R. "Digital Microfluidics for Immunoprecipitation" Analytical Chemistry, 2016, 88, 10223-10230. Supporting info.
  47. Choi, K.; Boyacı, E.; Kim, J.; Seale, B.; Barrera-Arbelaez, L.; Pawliszyn, J.; Wheeler, A.R. "A Digital Microfluidic Interface between Solid-Phase Microextraction and Liquid Chromatography–Mass Spectrometry" J. Chromatogr. A, 2016, 1444, 1-7. Supporting info.
  48. Zhang, B.; Montgomery, M.; Chamberlain, M.D.; Ogawa, S.; Korolj, A.; Pahnke, A.; A.Wells, L.; Massé, S.; Kim, J.; Reis, L.; Momen, A.; Nunes, S.S.; Wheeler, A.R.; Nanthakumar, K.; Keller, G.; Sefton, M.V.; Radisic, M. "Biodegradable Scaffold with Built-in Vasculature for Organ-on-a-Chip Engineering and Direct Surgical Anastomosis" Nature Materials, 2016, 15, 669-678.
  49. Yu, Y.; Shamsi, M.H.; Krastev, D.L.; Dryden, M.D.M.; Leung, Y.; Wheeler, A.R. "A Microfluidic Method for Dopamine Uptake Measurements in Dopaminergic Neurons" Lab Chip 2016, 16, 543-552. Supporting info
  50. Liu, C.; Choi, K.; Kang, Y.; Kim, J.; Fobel, C.; Seale, B.; Campbell, J.L.; Covey, T.R.; Wheeler, A.R. "Direct Interface between Digital Microfluidics and High Performance Liquid Chromatography–Mass Spectrometry" Analytical Chemistry, 2015, 87, 11967-11972. Supporting info.
  51. Shamsi, M.H.; Choi, K.; Ng, A.H.C.; Chamberlain, M.D.; Wheeler, A.R. "Electrochemiluminescence on Digital Microfluidics for MicroRNA Analysis" Biosensors and Bioelectronics, 2015, 77, 845-852. Supporting info.
  52. Dryden, M.D.M.; Wheeler, A.R. "DStat: A Versatile, Open-Source Potentiostat for Electroanalysis and Integration" PLoS ONE, 2015, 10, e0140349. Supporting info. Movie.
  53. Choi, K.; Mudrik, J.M.; Wheeler, A.R. "A Guiding Light: Spectroscopy on Digital Microfluidic Devices Using in-Plane Optical Fibre Waveguides" Anal. Bioanal. Chem. 2015, 407, 7467-7475. Supporting info.
  54. Rackus, D.G.; Dryden, M.D.M.; Lamanna, J.; Zaragoza, A.; Lam, B.; Kelley, S.O.; Wheeler, A.R. "A Digital Microfluidic Device with Integrated Nanostructured Microelectrodes for Electrochemical Immunoassays" Lab on a Chip, 2015, 15, 3776-3784. Supporting info.
  55. Ng, A.H.C.; Chamberlain, M.D.; Situ, H.; Lee, V.; Wheeler, A.R. "Digital microfluidic immunocytochemistry in single cells" Nat. Commun., 2015, 6, 7513. Supplementary: Info, Movie 1, Movie 2, Movie 3, Movie 4.
  56. Kim, J.; Abdulwahab, S.; Choi, K.; Lafreniere, N.M.; Mudrik, J.M.; Gomaa, H.; Ahmado, H.; Behan, L.-A.; Casper, R.F.; Wheeler, A.R. "A Microfluidic Technique for Quantification of Steroids in Core Needle Biopsies" Analytical Chemistry, 2015, 87, 4688-4695. Supporting info.
  57. Lafreniere, N.M.; Mudrik, J.M.; Ng, A.H.C.; Seale, B.; Spooner, N.; Wheeler, A.R. "Attractive Design: An Elution Solvent Optimization Platform for Magnetic-Bead-based Fractionation Using Digital Microfluidics and Design of Experiments" Analytical Chemistry, 2015, 87, 3902-3910.
  58. Ng, A.H.C.; Lee, M.; Choi, K.; Fischer, A.T.; Robinson, J.M.; Wheeler, A.R. "A Digital Microfluidic Platform for the Detection of Rubella Infection and Immunity: A Proof of Concept" Clin. Chem., 2015, 61, 420-429. Supporting info. Supporting video.
  59. Sarvothaman, M.K.; Kim, K.S.; Seale, B.; Brodersen, P.M.; Walker, G.C.; Wheeler, A.R. "Dynamic Fluoroalkyl Polyethylene Glycol Co-Polymers: A New Strategy for Reducing Protein Adhesion in Lab-on-a-Chip Devices" Adv. Func. Mater., 2015, 25, 506-515. Supporting info.
  60. Mei, N.; Seale, B.; Ng, A.H.C.; Wheeler, A.R.; Oleschuk, R. "Digital Microfluidic Platform for Human Plasma Protein Depletion" Analytical Chemistry, 2014, 86, 8466-8472. Supporting info. Supporting video.
  61. Au, S.H.; Chamberlain, M.D.; Mahesh, S.; Sefton, M.V.; Wheeler, A.R. "Hepatic organoids for microfluidic drug screening" Lab on a Chip, 2014, 14, 3290-3299. Supporting info.
  62. Shih, S.C.C.; Mufti, N.S.; Chamberlain, M.D.; Kim, J.; Wheeler, A.R. "A droplet-based screen for wavelength-dependent lipid production in algae" Energy & Environmental Science, 2014, 7, 2366-2375. Supporting info, supporting video.
  63. Kirby, A.E.; Lafrenière, N.M.; Seale, B.; Hendricks, P.I.; Cooks, R.G.; Wheeler, A.R. "Analysis on the Go: Quantitation of Drugs of Abuse in Dried Urine with Digital Microfluidics and Miniature Mass Spectrometry" Analytical Chemistry, 2014, 86, 6121-6129. Supporting video.
  64. Fobel, R.; Kirby, A.E.; Ng, A.H.C.; Farnood, R.R.; Wheeler, A.R. "Paper Microfluidics Goes Digital" Adv. Mat. 2014, 26, 2838-2843. Supporting info.
  65. Eydelnant, I.A.; Li, B.B.; Wheeler, A.R. "Microgels on-Demand" Nat. Commun. 2014, 5, 3355. Supplementary: Figures, Movie 1, Movie 2.
  66. Mudrik, J.M.; Dryden, M.D.M.; Lafrenière, N.M.; Wheeler, A.R. "Strong and small: strong cation-exchange solid-phase extractions using porous polymer monoliths on a digital microfluidic platform" Canadian Journal of Chemistry 2014, 92, 179-185.
  67. Lafrenière, N.M.; Shih, S.C.C.; Abu-Rabie, P.; Jebrail, M.J.; Spooner, N.; Wheeler, A.R. "Multiplexed extraction and quantitative analysis of pharmaceuticals from DBS samples using digital microfluidics" Bioanalysis 2014, 6, 307-318.
  68. Shamsi, M.H.; Choi, K.; Ng, A.H.C.; Wheeler, A.R. "A digital microfluidic electrochemical immunoassay" Lab on a Chip 2014, 14, 547-554.
  69. Choi, K.; Ng, A.H.C.; Fobel, R.; Chang-Yen, D.A.; Yarnell, L.E.; Pearson, E.L.; Oleksak, C.M.; Fischer, A.T.; Luoma, R.P.; Robinson, J.M.; Audet, J.; Wheeler, A.R. "Automated Digital Microfluidic Platform for Magnetic-Particle-Based Immunoassays with Optimization by Design of Experiments" Analytical Chemistry 2013, 85, 9638-9646. Supporting info.
  70. Dryden, M.D.M.; Rackus, D.G.; Shamsi, M.H.; Wheeler, A.R. "Integrated Digital Microfluidic Platform for Voltammetric Analysis" Analytical Chemistry 2013, 85, 8809-8816.
  71. Au, S.H.; Fobel, R.; Desai, S.P.; Voldman, J.; Wheeler, A.R. "Cellular bias on the microscale: probing the effects of digital microfluidic actuation on mammalian cell health, fitness and phenotype" Integr. Biol. 2013, 5, 1014. Supporting info.
  72. Kirby, A.E.; Wheeler, A.R. "Microfluidic Origami: A New Device Format for In-Line Reaction Monitoring by Nanoelectrospray Ionization Mass Spectrometry" Lab on a Chip 2013, 13, 2533-2540.
  73. Chen, M.B.; Srigunapalan, S.; Wheeler, A.R.; Simmons, C.A. "A 3D Microfluidic Platform Incorporating Methacrylated Gelatin Hydrogels to Study Physiological Cardiovascular Cell–Cell Interactions" Lab on a Chip 2013, 13, 2591-2598.
  74. Fobel, R.; Fobel, C.; Wheeler, A.R. "Dropbot: An Open-Source Digital Microfluidic Control System with Precise Control of Electrostatic Driving Force and Instantaneous Drop Velocity Measurement" Appl. Phys. Lett. 2013, 102, 193513. Supporting info.
  75. Shih, S.C.C.; Barbulovic-Nad, I.; Yang, X.; Fobel, R.; Wheeler, A.R. "Digital Microfluidics with Impedance Sensing for Integrated Cell Culture and Analysis" Biosens. Bioelectron. 2013, 42, 314–320. Supporting info
  76. Ng, A.H.C.; Choi, K.; Luoma, R.P.; Robinson, J.M.; Wheeler, A.R. "Digital Microfluidic Magnetic Separation for Particle-Based Immunoassays" Analytical Chemistry 2012, 84, 8805-8812. Supporting info
  77. Jebrail, M.J.; Assem, N.; Mudrik, J.M.; Dryden, M.D.M.; Lin, K.; Yudin, A.K.; Wheeler, A.R. "Combinatorial Synthesis of Peptidomimetics Using Digital Microfluidics" Journal of Flow Chemistry 2012, 2, 103-107.
  78. Shih, S.C.C.; Yang, H.; Jebrail, M.J.; Fobel, R.; McIntosh, N.; Al-Dirbashi, O.Y.; Chakraborty, P.; Wheeler, A.R. "Dried Blood Spot Analysis by Digital Microfluidics Coupled to Nanoelectrospray Ionization Mass Spectrometry" Analytical Chemistry 2012, 84, 3731-3738.
  79. Luk, V.N.; Fiddes, L.K.; Luk, V.M.; Kumacheva, E.; Wheeler, A.R. "Digital Microfluidic-Hydrogel Microreactors for Proteomics" Proteomics 2012, 12, 1310-1318.
  80. Fiddes, L.K.; Luk, V.N.; Au, S.H.; Ng, A.H.C.; Luk, V.M.; Kumacheva, E.; Wheeler, A.R. "Hydrogel Discs for Digital Microfluidics" Biomicrofluidics 2012, 6, 014112.
  81. Eydelnant, I.A.; Uddayasankar, U.; Li, B.; Liao, M.W.; Wheeler, A.R. "Virtual Microwells for Digital Microfluidic Reagent Dispensing and Cell Culture" Lab on a Chip 2012, 12, 750-757.
  82. Bogojevic, D.; Chamberlain, M.D.; Barbulovic-Nad, I.; Wheeler, A.R. "A Digital Microfluidic Method for Multiplexed Cell-Based Apoptosis Assays" Lab on a Chip 2012, 12, 627-634.
  83. Srigunapalan, S.; Eydelnant I.A.; Simmons, C.A.; Wheeler, A.R. "A Digital Microfluidic Platform for Primary Cell Culture and Analysis" Lab on a Chip 2012, 12, 369-375.
  84. Kuehne, A.J.C.; Gather, M.C.; Eydelnant I.A.; Yun, S.H.; Weitz, D.A.; Wheeler, A.R. "A Switchable Digital Microfluidic Droplet Dye-Laser" Lab on a Chip 2011, 11, 3716-3719.
  85. Jebrail, M.J.; Yang, H.; Mudrik, J.M.; Lafreniere, N.M.; McRoberts, C.; Al-Dirbashi, O.Y.; Fisher, L.; Chakraborty, P.; Wheeler, A.R. "A Digital Microfluidic Method for Dried Blood Spot Analysis" Lab on a Chip 2011, 11, 3218-3224. Supporting info.
  86. Au, S.H.; Kumar, P.; Wheeler, A.R. "A New Angle on Pluronic Additives: Advancing Droplets and Understanding in Digital Microfluidics" Langmuir 2011, 27, 8586-8594. Suppl. File 1.
  87. Yang, H.; Mudrik, J.M.; Jebrail, M.J.; Wheeler, A.R. "A Digital Microfluidic Method for in Situ Formation of Porous Polymer Monoliths with Application to Solid-Phase Extraction" Anal. Chem. 2011, 83, 3824-3830.
  88. Srigunapalan, S.; Lam, C.; Wheeler, A.R.; Simmons, C.A. "A Microfluidic Membrane Device to Mimic Critical Components of the Vascular Microenvironment" Biomicrofluidics, 2011, 5, 013409.
  89. Shih, S.C.C.; Fobel, R.; Kumar, P.; Wheeler, A.R. "A Feedback Control System for High-Fidelity Digital Microfluidics" Lab Chip, 2011, 11, 535-540. Suppl. File 1, Suppl. File 2.
  90. Au, S.H.; Shih, S.C.C.; Wheeler, A.R. "Integrated Microbioreactor for Culture and Analysis of Bacteria, Algae and Yeast" Biomed. Microdev., 2011, 13, 41-50.
  91. Miller, E.M.; Ng, A.H.C.; Uddayasankar, U.; Wheeler, A.R. "A Digital Microfluidic Approach to Heterogeneous Immunoassays" Anal. Bioanal. Chem., 2010, 399, 337-345.
  92. Kirby, A.E.; Jebrail, M.J., Yang, H.; Wheeler, A.R. "Folded Emitters for Nanoelectrospray Ionization Mass Spectrometry" Rapid Comm. Mass Spec., 2010 24, 3425–3431.
  93. Jebrail, M.J.; Ng, A.H.C.; Rai, V.; Hili, R.; Yudin, A.K.; Wheeler, A.R. "Synchonized Synthesis of Peptide-Based Macrocycles by Digital Microfluidics" Angew. Chemie. Int. Ed., 2010, 49, 8625-8629. Supporting Info
  94. Watson, M.W.L.; Jebrail, M.J.; Wheeler, A.R. "Multilayer Hybrid Microfluidics: A Digital-to-Channel Interface for Sample Processing and Separations" Anal. Chem. 2010, 82, 6680-6686.
  95. Barbulovic-Nad, I.; Au, S.; Wheeler, A.R. "A Microfluidic Platform for Complete Mammalian Cell Culture" Lab Chip 2010, 10, 1536-1542. Supporting Info 
  96. Fiddes, L.; Raz, N.; Srigunapalan, S.; Tumarkan, E.; Simmons, C.A.; Wheeler, A.R.; Kumacheva, E. "A Circular Cross-Section PDMS Microfluidics System for Replication of Cardiovascular Flow Conditions" Biomaterials 2010, 31, 3459-3464.
  97. Young, E.W.K.; Watson, M.W.L.; Srigunapalan, S.; Wheeler, A.R.; Simmons, C.A. "Technique for Real-Time Measurements of Endothelial Permeability in a Microfluidic Membrane Chip Using Laser-Induced Fluorescence Detection" Anal. Chem. 2010, 82, 808-816. Supporting Info
  98. Fiddes, L.; Chan, H.K.C.; Lau, B.; Kumacheva, E.; Wheeler, A.R. "Durable, Region-Specific Protein Patterning in Microfluidic Channels" Biomaterials 2009, 31, 315-320. 
  99. Jebrail, M.J.; Luk, V.N.; Shih, S.C.C.; Fobel, R.; Ng, A.; Yang, H.; Freire, S.L.S.; Wheeler, A.R. "Digital Microfluidics for Automated Proteomic Processing" J. Vis. Res. 2009, 33,http://www.jove.com/index/details.stp?id=1603
  100. Mousa, N.A.; Jebrail, M.J.; Yang, H.; Abdegawad, M.; Metalnikov, P.; Chen, J.; Wheeler, A.R.; Casper, R.F. "Droplet-Scale Estrogen Assays in Breast Tissue, Blood, and Serum" Sci. Trans. Med., 2009, 1, 1ra2. Supporting Info
  101. Abdelgawad, M., Park, P.; Wheeler, A.R. "Optimization of Device Geometry in Single-Plate Digital Microfluidics" J. App. Phys. 2009, 105, 094506.
  102. Luk, V.N.; Wheeler, A.R. "A Digital Microfluidic Approach to Proteomic Sample Processing" Anal. Chem. 2009, 81, 4524–4530 .
  103. Watson, M.W.L.; Mudrik, J.M.; Wheeler, A.R. "Gradient Elution in Microchannel Electrochromatography" Anal. Chem. 2009, 81, 3851-3857.
  104. Abdelgawad, M.; Watson, M.W.L.; Wheeler, A.R. "Hybrid Microfluidics: A Digital-to-Channel Interface for In-Line Sample Processing and Chemical Separations" Lab Chip 2009, 9, 1046-1051.
  105. Yang, H.; Luk, V.N.; Abdelgawad, M.; Barbulovic-Nad, I.; Wheeler, A.R. "A World-to-Chip Interface for Digital Microfluidics" Anal. Chem., 2009, 81, 1061-1067.
  106. Jebrail, M.; Wheeler, A.R. "A Digital Microfluidic Method for Protein Extraction by Precipitation" Anal. Chem. 2009, 81,  330-335.
  107. Fiddes, L.; Chan, H.K.C.; Wyss, K.; Simmons, C.A.; Kumacheva, E.; Wheeler, A.R. "Augmenting Microgel Flow Via Receptor-Ligand Binding in the Constrained Geometries of Microchannels" Lab Chip 2009, 9, 286-290.
  108. Abdelgawad, M.; Watson, M.W.L.; Young, E.W.K.; Mudrik, J.M.; Ungrin, M.D.; Wheeler, A.R. "Soft Lithography: Masters on Demand"  Lab Chip 2008, 8, 1379-1385. Supporting Info
  109. Luk, V.N.; Mo, G.C.; Wheeler, A.R. "Pluronic Additives: A Solution to Sticky Problems in Digital Microfluidics"  Langmuir 2008, 24, 6382-6389. Supporting Info
  110. Freire, S.L.S.; Yang, H.; Wheeler, A.R. "A Practical Interface for Microfluidics and Nanoelectrospray Mass Spectrometry"  Electrophoresis 2008, 29, 1836-1843.
  111. Abdelgawad M.; Freire S.; Yang H.; Wheeler A. R. "All-Terrain Droplet Actuation" Lab Chip 2008, 8, 672-677.  Supporting Info
  112. Barbulovic-Nad, I.; Yang, H.; Park, P.S.; Wheeler, A.R. "Digital Microfluidics for Cell-Based Assays" Lab Chip 2008, 8, 519-526.
  113. Miller, E.M.; Wheeler, A.R. "A Digital Microfluidic Approach to Homogeneous Enzyme Assays" Anal. Chem. 2008, 80, 1614-1619.
  114. Abdelgawad, M.; Wheeler, A.R. "Low-Cost, Rapid-Prototyping of Digital Microfluidics Devices"Microfl. and Nanofl. 2008, 4, 349-355.
  115. Young, E.W.K.; Wheeler, A.R.; Simmons, C.A. "Matrix-Dependent Adhesion of Vascular and Valvular Endothelial Cells in Microfluidic Channels" Lab Chip, 2007, 7, 1759-1756.
  116. Fiddes, L.; Young, E.W.K.; Kumacheva, E.; Wheeler, A.R. "Flow of Microgel Capsules through Topographically Patterned Microchannels" Lab Chip 2007, 7, 863-867.
  117. Qin, J.; Wheeler, A.R. "Maze Exploration and Learning in C. elegans" Lab Chip 2007, 7, 186-192.
  118. Abdelgawad, M.; Wheeler, A.R. "Rapid Prototyping in Copper Substrates for Digital Microfluidics" Adv. Mater. 2007, 19, 133-137.
  119. Watson, M.; Abdelgawad, M.; Ye, G.; Yonson, N.; Trottier, J.; Wheeler, A.R. "Micro-Contact Printing-Based Fabrication of Digital Microfluidic Devices" Anal. Chem. 2006, 78, 7877-7885.

Book Chapters and Review Papers:

Return to Primary Publications
  1. Bolotin, S.; Osman, S.; Halperin, S.; Severini, A.; Ward, B.J.; Sadarangani, M.; Hatchette, T.; Pebody, R.; Winter, A.; De Melker, H.; Wheeler, A.R.; Brown, D.; Tunis, M.; Crowcroft, N. "Immunity of Canadians and Risk of Epidemics Workshop – Conference Report" Vaccine, 2023, 41, 6775-6781.
  2. Stutzmann, C.; Peng, P.; Wu, Z.; Savole, C.; Sirois, I.; Thibault, P.; Wheeler, A.R.; Caron, E. "Unlocking the potential of microfluidics in mass spectrometry-based immunopeptidomics for tumor antigen discovery" Cell Press Methods, 2023, 3, 100511.
  3. Nemr, C.R.; Sklavounos, A.A.; Wheeler, A.R.; Kelley, S.O. "Digital Microfluidics as an Emerging Tool for Bacterial Protocols" SLAS Technology, 2023, 28, 2-15.
  4. Zhang, S.; Xu, B.; Elsayed, M.; Nan, F.; Liang, W.; Valley, J.K.; Liu, L.; Huang, Q.; Wuk, M.C.; Wheeler, A.R. "Optoelectronic tweezers: a versatile toolbox for nano-/micro-manipulation" Chem. Soc. Rev., 2022, 51, 9203–9242.
  5. Euliano, E.; Sklavounos, A.A.; Wheeler, A.R.; McHugh, K.J. "Translating Diagnostics and Drug Delivery Technologies to Low-Resource Settings" Sci. Transl. Med. 2022, 14, eabm1732.
  6. Zhong, J.; Riordon, J.; Wu, T.C.; Edwards, H.; Wheeler, A.R.; Pardee, K.; Aspuru-Guzik, A.; Sinton, D. "When Robotics Met Fluidics" Lab Chip 2020, 20, 709-716.
  7. Dryden, M.D.M.; Fobel, R.; Fobel, C.; Wheeler, A.R. "Upon the Shoulders of Giants: Open-Source Hardware and Software in Analytical Chemistry" Anal. Chem. 2017, 89, 4330−4338.
  8. Dixon, C.; Lamanna, J.; Wheeler, A.R. "Printed Microfluidics" Adv. Func. Mater. 2017, 27, 1604824.
  9. Ng, A.H.C.; Li, B.; Chamberlain, M.D.; Wheeler, A.R. "Digital Microfluidic Cell Culture" Annu. Rev. Biomed. Eng. 2015, 17, 91-112.
  10. Rackus, D.G.; Shamsi, M.H.; Wheeler, A.R. "Electrochemistry, Biosensors and Microfluidics: A Convergence of Fields" Chem. Soc. Rev. 2015, 44, 5320-5340.
  11. Kirby, A.E.; Wheeler, A.R. "Digital Microfluidics: An Emerging Sample Preparation Platform for Mass Spectrometry" Anal. Chem. 2013, 85, 6178-6184.
  12. Choi, K.; Ng, A.H.C.; Fobel, R.; Wheeler, A.R. "Digital Microfluidics" Annu. Rev. Anal. Chem. 2012, 5, 413–440.
  13. Jebrail, M.J.; Wheeler, A.R. "Let's Get Digital: Digitizing Chemical Biology with Microfluidics" Curr. Opin. Chem. Biol., 2010, 14, 574-581.
  14. Ng, A.H.C.; Uddayasankar, U.; Wheeler, A.R. "Immunoassays in Microfluidic Systems" Anal. and Bioanal. Chem. 2010, 397, 991-1007.
  15. Abdelgawad, M.; Wheeler, A.R. "The Digital Revolution: A New Paradigm for Microfludics" Adv.  Mat. 2009, 21, 920-925.
  16. Miller, E.M.; Wheeler, A.R. "Digital Bioanalysis" Anal. and Bioanal. Chem. 2009, 393, 419-426.
  17. Wheeler, A.R. "Chemistry: Putting Electrowetting to Work" Science 2008, 322, 539-540.
  18. Charon, L.; Wheeler, A.R.; Lilge, L. "Single-Cell Analysis in Microfluidics" In Encyclopedia of Micro- and Nanofluidics; Li, D. Q., Ed.; Springer: Heidelberg, Germany, 2008; Vol. 3, pp 1851-1859.
  19. Miller, E.M.; Freire, S.L.S.; Wheeler, A.R. "Proteomics in Microfluidic Devices" In Encyclopedia of Micro- and Nanofluidics; Li, D. Q., Ed.; Springer: Heidelberg, Germany, 2008; Vol. 3, pp 1749-1758.
  20. Freire, S.L.S.; Wheeler, A.R. "Interfaces between Microfluidics and Mass Spectrometry" In Encyclopedia of Micro- and Nanofluidics; Li, D. Q., Ed.; Springer: Heidelberg, Germany, 2008; Vol. 2, pp 849-854.
  21. Barbulovic-Nad, I.; Wheeler, A.R. "Cell Assays in Microfluidics" In Encyclopedia of Micro- and Nanofluidics; Li, D. Q., Ed.; Springer: Heidelberg, Germany, 2008; Vol. 1, pp 209-216.
  22. Barbulovic-Nad, I.; Lucente, M.; Sun, Y.; Wheeler, A.R.; Bussmann, Z. "Bio-Microarray Fabrication Techniques" Crit. Rev. Biotech. 2006, 26, 237-259.
  23. Freire, S.; Wheeler, A.R. "Proteome-on-a-Chip: Mirage, or on the Horizon?" Lab Chip 2006, 6, 1415-1423.