Digital microfluidics and hydrogels

Hydrogels are networks of hydrophilic polymer chains that are swollen with water, and they are useful for a wide range of applications because they provide stable niches for immobilizing proteins and cells. Hydrogels have very recently been combined with digital microfluidics: and systematically addressed by droplets of reagents. The combination of hydrogels and digital microfluidics is an exciting new area for a wide range of applications; papers describing our efforts in this area are listed below.

Li, B.B.; Scott, E.Y.; Olafsen, N.E.; Matthews, J.; Wheeler, A.R. "Analysis of the Effects of Aryl Hydrocarbon Receptor Expression on Cancer Cell Invasion via Three-Dimensional Microfluidic Invasion Assays" Lab Chip 2022, 22, 313–325. Supporting Info.

Li, B.B.; Scott, E.Y.; Chamberlain, M.D.; Duong, B.T.V.; Zhang, S.; Done, S.J.; Wheeler, A.R. "Cell Invasion in Digital Microfluidic Microgel Systems" Science Advances. 2020, 6, eaba9589. Supporting Info.

Au, S.H.; Chamberlain, M.D.; Mahesh, S.; Sefton, M.V.; Wheeler, A.R. "Hepatic organoids for microfluidic drug screening" Lab on a Chip, 2014, 14, 3290-3299. Supporting info.

Eydelnant, I.A.; Li, B.B.; Wheeler, A.R. "Microgels on-DemandNat. Commun. 2014, 5, 3355. Supplementary: Figures, Movie 1, Movie 2.

Luk, V.N.; Fiddes, L.K.; Luk, V.M.; Kumacheva, E.; Wheeler, A.R. "Digital Microfluidic-Hydrogel Microreactors for Proteomics" Proteomics 2012, 12, 1310-1318.

Fiddes, L.K.; Luk, V.N.; Au, S.H.; Ng, A.H.C.; Luk, V.M.; Kumacheva, E.; Wheeler, A.R. "Hydrogel Discs for Digital Microfluidics" Biomicrofluidics 2012, 6, 014112.