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Unverified Commit afb8ee48 authored by Erica Y. Scott's avatar Erica Y. Scott Committed by GitHub
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# DISCO: scRNAseq ![alt text](https://github.com/eyscott/DISCO_scRNAseq/blob/master/Images/WheelerLabLogo.png "Wheeler Lab")
# DISCO: scRNAseq <img src="https://github.com/eyscott/DISCO_scRNAseq/blob/master/Images/WheelerLabLogo.png" alt="Wheeler Microfluidics Lab" width="50" height="50"/>
DISCO-Digital Microfluidic Isolation of Single Cells for - Omics.
The laser lysis capture method implemented in DISCO offers spatially and temporally resolved capture of single cells, while Digital Microfluidics (DMF) is capable of retrieving this lysate and permitting flexible downstream analyses, such as scRNA-seq.
Each cell has a barcode incorporated into Read 1, making the cell's transcriptome accountable and traceable throughout the pipeline. This barcode includes a unique cell barcode (12bp) and a UMI (8bp). The first part of our bioinformatic pipeline deals with parsing these.
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